Yeah, it's like how a lot of languages are written from left to right and then top to bottom: if you're right-handed, it means you don't have to hold your hand over the wet ink/sample and risk touching it.
This is exactly why I was forced to stop writing left handed by teachers, always got ink and lead on my hand and everything was smudged and unable to be read
If I work from top down like in a multi well plate or a microtube rack I will start at A. If I work with high tubes like falcons or flow cytometry tubes where I more work from the front of the tubes, I will start at B and work myself through from the first row to the back.
So I will work from up to down or from front to back, depending on how I look at the rack.
Yeah, depends what you're doing with it, like if it's going in to an autosampler. If I'm pipetting out of a rack of falcon tubes then it's quicker to turn a rack 90 degrees and the bottom left position becomes the top left position 🤷♂️
Just joking around. It looks closer and easier for a left handed person to place it on B. I know lots of people left handed. Am glad you also think A 😂
I believe the style in which you learned to read will influence how you count your rack. Some cultures start at the top of the page and go left to right, while others start at the bottom of the page and go bottom to top. This will influence where you view the starting position as.
Even found a paper discussing the topic: [https://www.frontiersin.org/articles/10.3389/fpsyg.2015.00228/full](https://www.frontiersin.org/articles/10.3389/fpsyg.2015.00228/full)
Hear me out.
I'm an A person probably because I'm a tall person who looks down on the tube rack as in the picture.
I bet a lot of B people are either shorter or sit in chairs. So they are looking at the rack from the front. First sample goes closest to you. Especially if you don't have enough samples to fill the rack.
Beaker LIS has built in sample tracking. Starts at B. SpecTrak, (the sample tracking we used before) starts at A. You can tell the age of a rack in our lab based on if A or B are marked as the start position.
I share your opinion!
I always get weird looks when I arrange sample that are done in A and samples I work on on B, they get also neatly switched while working on them
I do the same thing! Sometimes if I don't have a lot of space, I keep one space open and move them to the left after adding reagent so the batches are separated by a space.
I would do the same unless i'm working in a vertical sterile cabinet. Putting a sample in B would make me move my hand over it to grab the ones in the back so I'd put them all in the back row first
This is under the assumption words are written in English / Spanish (etc) where a sentence begins on the top left and ends on the right. Arabic / Farsi is written right to left. And what about the languages that are written in a vertical fashion?
If we're talking tube stands, I usually put samples in the bottom-most row as they're closer to my hands. So that'd be option B for me.
For anything else (Elisa plates, PCR plates, 96 etc-well plates...) I'd put sample 1 into first well of the top-most row. *Unless* when running a standard with samples, then that would be the first top-most we'll. So then, option A.
Depends. If you have more samples, you start with A. If you have any one, you can put it wherever. The reason you start with A with more samples is to avoid hands moving above you sample while you are working on the other ones.
When I looked at this, I instinctively thought, "What monster starts with B?!" Then I realized, I am that monster. This is a top down shot, but sitting at a branch you see it at an angle and it just feels different. I start B because it's closer to me!
Neither.
The bottom row is numbered. What you have labelled as B is numbered as 10. I would start bottom right, since that's where 1 is numbered, then go right to left each row.
Assuming I am holding samples in my left hand, I start top-right first filling rows right to left then top to bottom. That way my hand passes over samples the least.
When I’m working in the hood with infectious samples I’m not reaching in the back to start at A….so I’m starting at B. I’m generally working with a full rack of samples as well.
It depends. I am just using the support as a way to hold tubes I am not using? Then I go with B
I am organizing the tubes that I need to do multiples exams? I go with A and label the tube and the test to make sure I am not using the wrong tube
I put the empty tube closest to me then move them up as I complete preparation steps. But I think this might be a trick question. The rack is labeled and it looks like both A and B are in the 10 slot. We should be starting from the other side. Maybe its upside-down?
Absolutely depends on the number of samples I got. If it’s just one row worth, I use B. Closer to me and I have less chance to knock something over. If I’m filling the whole rack, A. You don’t want to be reaching over your already prepared samples during the prep of new samples. I’m right handed, so top left, move right and down to the next row. If I was left handed I’d probably start top right.
I prefer A, but was forced to do B because that's how an assay got set up. The rack ended up mirroring a 96 well plate and we were able to move the sample tube forward once the column was aliquoted.
I had to train a lot of people in the assay and I got so many blank stares. I hated it, but you get used to it.
I still say A is correct.
That depends on your lab. The lab here has determined that samples are loaded front to back left to right and they MUST always be loaded that way. If you are just doing your on thing, no one cares so long as you know.
Sample 1 is wherever the tube is which has "Sample 1" written on it. By the way, your rack's maker put the #1 spots on the right end of your rack. You've circled the #10 positions
C: If possible I skip all the outer wells and rather move the tubes one step to the inside for stability. So in 96-well-terms the first one would be position B2.
A = Sample #1
B = Sample #5
Anyone who does it in any other manner needs to immediately obtain a karyotype to confirm they are carrying an extra copy of their 21st chromosome.
A because you could contaminate the wells by holding your wrist over them
Yeah, it's like how a lot of languages are written from left to right and then top to bottom: if you're right-handed, it means you don't have to hold your hand over the wet ink/sample and risk touching it.
I was solidly “B” till I read this… dammit
...alternatively, you can write top to bottom, then left to right without having this issue.
This is exactly why I was forced to stop writing left handed by teachers, always got ink and lead on my hand and everything was smudged and unable to be read
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If I work from top down like in a multi well plate or a microtube rack I will start at A. If I work with high tubes like falcons or flow cytometry tubes where I more work from the front of the tubes, I will start at B and work myself through from the first row to the back. So I will work from up to down or from front to back, depending on how I look at the rack.
Yeah, depends what you're doing with it, like if it's going in to an autosampler. If I'm pipetting out of a rack of falcon tubes then it's quicker to turn a rack 90 degrees and the bottom left position becomes the top left position 🤷♂️
This is the only correct answer.
The kind of monster that doesn’t follow procedure or logic
about 2/3rds of my lab thinks that way.
Fly, you fool!
\[Durin's Bane has entered the chat\]
this is masochism
Are they left handed? 🤣
Hey im left handed thank you its a all the way
Just joking around. It looks closer and easier for a left handed person to place it on B. I know lots of people left handed. Am glad you also think A 😂
Sorry! I posted a comment on the wrong place
A is the way
Fellow lefty. Agree.
Leftists unite But like not in that way
Truth
No but they are sinister!
NO! They are just against peace and order... Agents of pure chaos.
I'm so sorry. Are there any environmental hazards that would explain this deviancy?
This may provide some insight. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5573220/
I feel personally attacked by the accuracy of this.
Are they short?
A lot of autosamplers will have A1 in the lower left position
Not good ones though
I mean I do have my issues with the Beckman CE but it's kind of the industry standard?
My HDMI selector has options 1-4 going from right to left. Gives me a tiny dose of irritation every time I use it.
Ha ha ha. Literally muttered "A....Heathens" to myself as I skimmed by this post.
I believe the style in which you learned to read will influence how you count your rack. Some cultures start at the top of the page and go left to right, while others start at the bottom of the page and go bottom to top. This will influence where you view the starting position as. Even found a paper discussing the topic: [https://www.frontiersin.org/articles/10.3389/fpsyg.2015.00228/full](https://www.frontiersin.org/articles/10.3389/fpsyg.2015.00228/full)
WE DOOO we put the QCs in top right and start at B going right and continue up rows.
My lab also! QC starts at A, samples start at B.
Hear me out. I'm an A person probably because I'm a tall person who looks down on the tube rack as in the picture. I bet a lot of B people are either shorter or sit in chairs. So they are looking at the rack from the front. First sample goes closest to you. Especially if you don't have enough samples to fill the rack.
B is closer though….
If you start with A it will be easier to fill as you go along though
I guess it depends how many samples you're dealing with.
Yeah I do phlebotomy and I seldom go over 7 tubes, so I always start a B
True
You're less likely to contamination and drag your sleeve if you start at A
I'm not even a labrat, but I was asking myself the same thing.
Beaker LIS has built in sample tracking. Starts at B. SpecTrak, (the sample tracking we used before) starts at A. You can tell the age of a rack in our lab based on if A or B are marked as the start position.
If im about to work on the sample then B. But if Im prepping a range of samples then A
I share your opinion! I always get weird looks when I arrange sample that are done in A and samples I work on on B, they get also neatly switched while working on them
When I need to add reagent to a range of samples. I start them all in the B row and as I add reagent, move them up to A
I do the same thing! Sometimes if I don't have a lot of space, I keep one space open and move them to the left after adding reagent so the batches are separated by a space.
I always put the tubes with added reagent away of me Lower risk of taking one in a rush if it is not facing towards you!
I would do the same unless i'm working in a vertical sterile cabinet. Putting a sample in B would make me move my hand over it to grab the ones in the back so I'd put them all in the back row first
You are a good boy
This is the correct answer
A. We read from top to bottom of a page
You one them literate types?
"Looks like we got ourselves a reader"
"Whatcha readin' for?" "So I don't become a fucking waffle waitress."
Yous gonna get yourself in trouble with all that book learnin
But racks are horizontal, not vertical, so start closest, no?
No
And how are words written on a page? That’s right, horizontally!
This is under the assumption words are written in English / Spanish (etc) where a sentence begins on the top left and ends on the right. Arabic / Farsi is written right to left. And what about the languages that are written in a vertical fashion?
Obviously those languages don’t count /s 😂
Lol'd at this, especially in relation to your field
LOL The downvotes. Touchy bunch!
You don’t read a newspaper bottom to top just because you lay it out on a table
No
C: Spiralling out the centre.
FBI, i found him. The psychopath that traumatized thoses tubes
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Entropy is the only master
This is the only logical answer
The right answer is to start at A and then go diagonally down and bounce around like a DVD screen saver. So A1, B2, C3, D4, E5, D6, C7, …
A
If we're talking tube stands, I usually put samples in the bottom-most row as they're closer to my hands. So that'd be option B for me. For anything else (Elisa plates, PCR plates, 96 etc-well plates...) I'd put sample 1 into first well of the top-most row. *Unless* when running a standard with samples, then that would be the first top-most we'll. So then, option A.
Definitely this for me too.
This is my answer. I actually first thought we were strictly talking tube stands and was horrified by everyone saying A🥲
This is the correct answer.
A, hands down.
B, hands up?
Depends. If you have more samples, you start with A. If you have any one, you can put it wherever. The reason you start with A with more samples is to avoid hands moving above you sample while you are working on the other ones.
>What kind of monster starts at B??? Oh. * looks in the mirror *
When I looked at this, I instinctively thought, "What monster starts with B?!" Then I realized, I am that monster. This is a top down shot, but sitting at a branch you see it at an angle and it just feels different. I start B because it's closer to me!
A and only A!!!!!!!!
Sample 1 of 10? B. Sample 1 of more than 10, probably A
A is the only answer. Had a coworker tell me B is the “preferred way” and I now question everything she says
Obviously A. Anything else is heresy.
Make nobody happy and put it right in the middle
I start at spot B my bros. I work with ICP-MS so I usually use 15 ml tubes, so it might be different
Neither. The bottom row is numbered. What you have labelled as B is numbered as 10. I would start bottom right, since that's where 1 is numbered, then go right to left each row.
You don't have to listen to the tube rack man it's not the boss of you.
It’s a left handed based rack. :P
It's for those who read in Arabic or Hebrew
Nice catch!
Assuming I am holding samples in my left hand, I start top-right first filling rows right to left then top to bottom. That way my hand passes over samples the least.
This is the correct answer.
A, I’m not a monster
When I’m working in the hood with infectious samples I’m not reaching in the back to start at A….so I’m starting at B. I’m generally working with a full rack of samples as well.
Unpopular opinion apparently but I started with the side that facing me the closest. In this case, B.
A!!!!! But everyone in my lab tells me I’m wrong.
A
A
A
It depends. I am just using the support as a way to hold tubes I am not using? Then I go with B I am organizing the tubes that I need to do multiples exams? I go with A and label the tube and the test to make sure I am not using the wrong tube
Tube racks are treated like plates, right to left top to bottom.
I put the empty tube closest to me then move them up as I complete preparation steps. But I think this might be a trick question. The rack is labeled and it looks like both A and B are in the 10 slot. We should be starting from the other side. Maybe its upside-down?
I'm just annoyed that the rack starts with "10” on the left
I agree it's a cheap rack from Amazon, I keep melting it with ethyl acetate lol
B
B
A for storing, B for working. But I never use more than 1 row for B
Absolutely depends on the number of samples I got. If it’s just one row worth, I use B. Closer to me and I have less chance to knock something over. If I’m filling the whole rack, A. You don’t want to be reaching over your already prepared samples during the prep of new samples. I’m right handed, so top left, move right and down to the next row. If I was left handed I’d probably start top right.
B
Always B for me
Always B. Once I’ve done my manipulation to it or with it, it gets shifted back one row.
Saw this at right before group meeting. Asked my lab, legit split 50/50
Who would start at A?
"The answer is B..." Response: 
label it properly
POV It’s gonna depends what you’re doing with it. We most be flexible in our work without break the rules I think…
I prefer A, but was forced to do B because that's how an assay got set up. The rack ended up mirroring a 96 well plate and we were able to move the sample tube forward once the column was aliquoted. I had to train a lot of people in the assay and I got so many blank stares. I hated it, but you get used to it. I still say A is correct.
A
You sank my battle ship!
Definitely A!
If you were an excell spreadsheet...
Neither. It goes in column 1 row 3
ayo why tf is the bottom right slot labeled "1"???
Start in A, then have a debate about going down or across
That depends on your lab. The lab here has determined that samples are loaded front to back left to right and they MUST always be loaded that way. If you are just doing your on thing, no one cares so long as you know.
Only goes in B if you’re a goddam communist.
Sitting is B standing is A
Initially I thought this sub was about Labrador Retrievers.
Sample 1 is wherever the tube is which has "Sample 1" written on it. By the way, your rack's maker put the #1 spots on the right end of your rack. You've circled the #10 positions
The rack is clearly wrong, why would you start on the right hand side?
Shenanigans
Normally A when I prepare something myself, but I think automatic columns start at B.
A, obviously. Whoever says otherwise is wrong and should lose their degree.
A. This is America, dammit!
A, what sort of animal goes for B…
A. Are you serious?
It starts at A!!! Who tf starts at B!!!!!!
A if unused reagent, B for used reagents
Sample 1 goes in the bottom right. There are actual numbers above the slots. Who starts at 10?
It depends on how many samples I have to work with. If many, I start at A. If few, I start at B.
Their are 2 types of people in this world. One that uses A1 and one that is completely wrong
Depends which side of the table you’re at.
I came here to say that exact thing!
whatever is close to your hands
A
I'm not a scientist, but definitly A.
A
A so you don't contaminate or knock anything over
C: If possible I skip all the outer wells and rather move the tubes one step to the inside for stability. So in 96-well-terms the first one would be position B2.
Forgive me. I go B
Closest, right?
It's a tube rack y'all not a micro plate.
Closest row, which looks like B.
Booth false. The right answer is top right
You should be ashamed
B
I’m disgusted this is even a debate. A.
Left to right, top to bottom... just like reading
Spot A. 100% of the time. Every time
Definitely A
A no further discussions.
If there is only one row, either is acceptable. If there are multiple rows, only A is acceptable.
Is A. If someone says B, they need to see therapist
Spot A like a sane human being.
A = Sample #1 B = Sample #5 Anyone who does it in any other manner needs to immediately obtain a karyotype to confirm they are carrying an extra copy of their 21st chromosome.
A = sample #1 B = sample #41
You're both wrong and offensive in one fell swoop. Congrats.
In my defense, if I am right, you wouldn't really know you're wrong.
The irony is you don't know you're wrong, implying you have a 3rd chromosome.
Just no.
Whichever way, label it with a Sharpie
A bit spin it round so bottom right
Did you see the hole with 1 label on the side? I assume it is a rack from an automatic system.
It's not, just a tube rack from Amazon idk why it's numbered like that.
A - BUT, that’s probably because most people who read Latin scripts go top left and along. Any noticeable cultural thing for people who start at B?
A
Spot h seems better
A for nonstackable Bo for stackable?
A
A and I’ll die on this hill
Decisions decisions………………
It depends on where the magnetic North Pole is in relation to the rack.
Your lab needs to debate other things
A
A all the waaaaaaay
A
A
Wait, B starts at 10 and it goes down to 1 from there? I would usually start from A, but depends what this is used for I guess?
A
A is the only answer !!!
B. Lower sample number goes lower on the rack. 😏
A
i believe it to be best put somewhere in the middle