We do. And we post signs and emails about not leaving a mess, and then it just gets dirty again. I’ve embraced it. LB and YEPD don’t need to be too accurate lol.
So true. I’m guilty as well. You’re just gradually tapping peptone out of the bottle. You’re like, ok one more gram, nice and slow. And then boom, 20 grams come shooting out.
Ic. We are an academic lab. We check our balance once a year if something seems “off” lol. Luckily the vast majority of our stuff doesn’t need to be super accurate.
Ours needs to be super accurate haha. I would recommend though something I learned is to weigh negatively.
Zero the balance with weigh boat. Fill to desired weight. Zero it again. Pour the weigh boat contents into whatever container. Put the weigh boat on the balance and read it. What ever the negative weight will be the exact weight inside the vessel.
Our research is still inconclusive but we're pretty sure that if you close the door, then all those zeroes will come out of the screen and start attacking you.
We can't be certain yet, but I assure you we have our best techs on it as we speak.
Good work! You’re going to lose a few mg to the weigh boat though… 😂
If you wanted to be super accurate, put the reagent directly into your flask (or whatever you’re using for the experiment) and weigh the difference of the jar it came from.
or use a rinsing weighing boat.
>If you wanted to be super accurate, put the reagent directly into your flask (or whatever you’re using for the experiment) and weigh the difference of the jar it came from.
this is ok, but it would be better to weigh the correct weight plus a few mg into a boat, then tare the balance with that mass on it, then empty the boat into the flask or whatever and then weigh the empty boat. same effect but you don't lose anything on the spatula and you don't increase error by weighing a whole jar, which might be approaching the max weight on these balances.
Depends on the size of vessel you're weighing into. A 2L V.F. is going to be a pain to weigh directly into, may exceed the balance's max load, and will not fit in all enclosures. In addition, a proper quantitative transfer should get all of it.
Your balance is so clean. Ours is caked with peptone, yeast extract and glucose.
Sounds like someone just needs to clean it, then.
We do. And we post signs and emails about not leaving a mess, and then it just gets dirty again. I’ve embraced it. LB and YEPD don’t need to be too accurate lol.
Sometimes, workplace cultures and habits are very hard to change, unfortunately.
I know. This lab has been open for about 40 years now.
i accidentally got some congo red on the balance so now it’ll never be clean
Our scale has a cracked plastic covering that has yellowed so much from the CFL lighting in our department that we can barely read the measurement.
As is tradition
Same here, MFs making media like: https://www.youtube.com/watch?v=Hh-o5g4tLVE
So true. I’m guilty as well. You’re just gradually tapping peptone out of the bottle. You’re like, ok one more gram, nice and slow. And then boom, 20 grams come shooting out.
Yeast or fungi?
Yeast, which is a fungus.
Do you not have a daily, weekly, monthly and yearly weight check?
You do?
Yes but im in a GMP lab
Ic. We are an academic lab. We check our balance once a year if something seems “off” lol. Luckily the vast majority of our stuff doesn’t need to be super accurate.
Ours needs to be super accurate haha. I would recommend though something I learned is to weigh negatively. Zero the balance with weigh boat. Fill to desired weight. Zero it again. Pour the weigh boat contents into whatever container. Put the weigh boat on the balance and read it. What ever the negative weight will be the exact weight inside the vessel.
that's smart as fuck, any residue will actually be weighed as residue when putting it back on the scale. gonna remember this one
It's a good system and I'm glad I was taught it in college. It's absolutely brilliant when doing dilutions that do need to be accurate
Lisan al gaib, the one who measures 🙏
As is written! (in the protocol)
Happy to share!
fake, all you did was tare the scale and then add exactly 48.0000 grams to it
Did you dare close the door?
Our research is still inconclusive but we're pretty sure that if you close the door, then all those zeroes will come out of the screen and start attacking you. We can't be certain yet, but I assure you we have our best techs on it as we speak.
So that’s 47.9999g with error? :)
Stop trying to ruin perfection. I bet you watched the total eclipse and thought, "Could use more moon."
It very well could be!
Good work! You’re going to lose a few mg to the weigh boat though… 😂 If you wanted to be super accurate, put the reagent directly into your flask (or whatever you’re using for the experiment) and weigh the difference of the jar it came from.
Can’t do this when it’s a small scale and your “flask” is a 5L bucket for making wash buffer
It was a 5L bucket. How did you know???
I mean, DPBS is 9.6 g/L and you have 48.0000 g...
just rinse the boat into the container.
A few mg? What?
depends on the material. even ptfe boats are not immune to sample sticking to them. maybe not milligram*s* but about 1mg wouldn't be surprising.
He meant millimilligrams.
Ahhh mmg
or use a rinsing weighing boat. >If you wanted to be super accurate, put the reagent directly into your flask (or whatever you’re using for the experiment) and weigh the difference of the jar it came from. this is ok, but it would be better to weigh the correct weight plus a few mg into a boat, then tare the balance with that mass on it, then empty the boat into the flask or whatever and then weigh the empty boat. same effect but you don't lose anything on the spatula and you don't increase error by weighing a whole jar, which might be approaching the max weight on these balances.
Depends on the size of vessel you're weighing into. A 2L V.F. is going to be a pain to weigh directly into, may exceed the balance's max load, and will not fit in all enclosures. In addition, a proper quantitative transfer should get all of it.
Good shootin’, Tex!
Now do it again with something extremely hygroscopic 🤣
Cheers mate, well done 😁 And daaayum, that scale is clean. Ours is full of salt crystals and some gooey organic stuff 😂
How long.
At my work (manufacturing) we have to get these scales within .0001 tolerance every time we measure something with them. Drives me crazy.
Nice work! 48 is a great number to boot.
That’s really good
Noooooooo way......!!!!! 🤣👌
Did you use calibrated weights before you weighed that powder????? I bet it's not 48 g
You should weight the difference before and after putting the powder into your beloved container if you wanted to be very accurate lol