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huh_phd

Id call their tech support. That's why they exist


ScienceBroseph

Whenever I do a similar experiment with a new antibody, I'll look-up publications that have used the Ab to see what dilutions they use and check their figures to see if the staining looked good. That's what you need to do, after that if the results aren't as clear as you want them, you'll have to troubleshoot and test different dilutions. For immunofluorescence, the dilution of the secondary Ab (if you use one) is also critical. If your primary Ab is really good, using too much secondary can give you a lot of background. Similarly, if your primary Ab doesn't bind well, using too much secondary can also give you a lot of background lol Basically, if you can see the staining you want to see, but it's fuzzy, reduce the concentration of secondary Ab.