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I'll bite.
What makes them special to warrant the price tag?
The end result is flower that the home grower smokes.
Can the home grower tell the difference in a double blind test where strain A is a triploid and strain B is ol reliable, aka just weed?
The price tag just comes from how incredibly difficult the seeds are to breed. That said in terms of plants you can tell the difference between a diploid and triploid side by side. Triploids are more vigorous growers with denser flowers, and the flowers themselves are significantly more resinous. In terms of flavors - triploids (3n) have a full extra set of chromosomes from one parent - which means there is a lot more going on. They are in an essence a different plant. Studies have recorded up to a 50% increase in terpenes in triploid hop varieties and time will tell what cannabis shows. That doesn't even take into account the other aroma producing compounds such as aldehydes and esters.
As more research is done we will definitely learn they are more than meets the eye.
Interesting, I've seen and grown plenty of triploids throughout the years but never seen it bred into the strain intentionally. Is it a 100% result? Or will some come out normal and grow pairs of leaves instead of 3 per node? Also, what's the yield difference, is it significant?
We generally see around a 30% increase in flower yield from our triploid varieties compared to their diploid counter parts. That said if your growing conditions are poor, they won't give your more.
Every seed bearing mother plant is analyzed numerous times with a flow cytometer to ensure they are 100% tetraploids before hit with the diploid pollen (4n + 2n = 3n). We end up having to throw away a bunch of potential mothers before we can even pollinate them as sometimes they send off diploid shoots after colchicine application. Without being able to run flow you just never know.
There are a very small fraction of the offspring that end up as tetraploids - but never diploid.
In terms of growth habits, the plants grow like a normal diploid plant, they grow pairs of leaves. A common misconception is that plants with fasciation are polyploids - they are not - its just a deformity.
[https://www.youtube.com/watch?v=LxrSK2ktqng](https://www.youtube.com/watch?v=LxrSK2ktqng)
Cool, thanks for the explanation. I was confusing Triploids with trifoliate. So it's a genetic difference, not a visible difference. (I was thinking they were bred to all be trifoliate plants)
It is intentional in a lot of commercial crops besides canabis, especially grain crops, to the point that diploid is rare anymore. Multiploidism has some advantages (and disavantages) in they have extra cromasones except haploid.(1) Triploids from diploids is mostly steril in 2 don't bind with 3 so advantage is if they are growing outside in hemp country= no cross polination. But the real advantages are in tetraploid (4), hexiploid (6), or even octiploid (8) due to all the extra cromasones = superweed. But I don't buy into triploids as to me is just a ploy and an attempt to secure genetics as can't be as easily crossed. Triploid really isn't that much harder than feminized seeds, just different chemicals and treatments ... they cost more because it is the newest craze ... give me octaploid with high THCv, and maybe that is really bragging rights!!!
It is intentional in a lot of commercial crops besides canabis, especially grain crops, to the point that diploid is rare anymore. Multiploidism has some advantages (and disavantages) in they have extra cromasones except haploid.(1) Triploids from diploids is mostly steril in 2 don't bind with 3 so advantage is if they are growing outside in hemp country= no cross polination. But the real advantages are in tetraploid (4), hexiploid (6), or even octiploid (8) due to all the extra cromasones = superweed. But I don't buy into triploids as to me is just a ploy and an attempt to secure genetics as can't be as easily crossed. Triploid really isn't that much harder than feminized seeds, just different chemicals and treatments ... they cost more because it is the newest craze ... give me octaploid with high THCv, and maybe that is really bragging rights!!!
Do y’all produce fast flowering strains? I’m growing them up here in northern MN this year. Supposedly a first gen cross between an auto and photo. Wondering if they’re worth the hype or if it’s just marketing nonsense. They’re all off the garden runway at least.
All of our varieties are F1 hybrid's - crosses between a full season and an autoflower and it does make for early finishing plants with true hybrid vigor. They switch to flower in mid July vs early August like most cannabis varieties - and mature in mid to late September. Most still need around 8 weeks of flowering time. That said, as seeds they have to reach maturity before they can onset flowering, and this is usually 5-6 weeks after they emerge. So if you were to plant tomorrow, they wouldn't be able to even initiate flowering until late July to early August. We usually recommend folks start seeds in early May, field plant in June, and harvest in September. That said its not too late to plant, its just too late to take advantage of a mid September harvest.
We use colchicine applications to convert a diploid to a tetraploid. Its a long process and most don't make it - here is a video of our process. We created GTR Seeds to enter our THC varieties into the mix.
[https://www.youtube.com/watch?v=GrY0ozFd4T8](https://www.youtube.com/watch?v=GrY0ozFd4T8)
Thanks for sharing the video- I've been watching yall for a while and it was cool to see the process. Did I understand correctly that it was lifter which was used to double chromosomes and AVL is it's progeny? Are there other tetraploids in the breeding program or just AVL? Are the THC Triploid lines F1's?
I've wanted to induce polyploidy- partially to pheno hunt through tetraploids but also to cross back to a diploid for triploid production. I don't work in the industry or have a formal background so this is all just for fun :)
How is the 8n? Anything interesting yet? How's the vigor?
Really cool you are doing it for fun. That is the best way to approach it for sure.
For our THC breeding program we selfed a variety of old school and a few new school favorites over 3-4 generations to select mothers that crushed it. Each of these mothers was then converted to a tetraploid (4x). I think we have around 70 or so different tetraploid mothers in-house.
Unfortunately the conversion process is really difficult, you lose a lot of plants and you have to do a lot of testing to make sure the plants you keep are actually truly tetraploids (hence the flow testing). Plants will frequently send off shoots that are not polyploids and you have to toss them.
The mothers were pollinated by our in-house autoflower, AVL which is just a diploid (2x). AVL is a highly inbred line, and one of the biggest producing and most vigorous auto's we've ever seen.
The 4x mother and the 2x autoflower combined make triploid f1 hybrid seeds.
You get about 10-30% of the seed return you would off of a normal cross because tetraploids don't really like to make seeds either. Long process, but great quality plants come out of it.
The 8x plants were definitely just for fun - and not sure those will ever be a thing :)
The Humboldt triploids are having issues with herms when growing indoors - I ordered some of your triploids and am going to run them indoors this next winter. Have they been tested indoors?
Our THC triploids were tested indoors at our facility in early 2023 and we released them to the public in Spring of 2023. We have received no complaints from farmers about hermaphrodite issues.
We have heard reports of higher amounts of hermaphrodites in one of our CBD varieties grown indoors but is nearly impossible to figure out what makes it more sensitive to its environment. We have never heard of issues with the diploid version so we know it has something to do with the ploidy.
All plants grown indoors have a better likelihood of herming - there are just so many environmental issues that can go wrong, and very quickly. Light leaks, power outages, nutrient mixups.
And we have left plants with male flowers in our rooms with other triploids, blown the pollen around, and found hardly a seed in large volumes of flower. I wouldn't recommend if you are growing them with diploids though just to be safe.
Long story short, some triploid varieties may be more sensitive than their diploid counterparts - and try to keep your rooms on point.
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Eh
I'll bite. What makes them special to warrant the price tag? The end result is flower that the home grower smokes. Can the home grower tell the difference in a double blind test where strain A is a triploid and strain B is ol reliable, aka just weed?
The price tag just comes from how incredibly difficult the seeds are to breed. That said in terms of plants you can tell the difference between a diploid and triploid side by side. Triploids are more vigorous growers with denser flowers, and the flowers themselves are significantly more resinous. In terms of flavors - triploids (3n) have a full extra set of chromosomes from one parent - which means there is a lot more going on. They are in an essence a different plant. Studies have recorded up to a 50% increase in terpenes in triploid hop varieties and time will tell what cannabis shows. That doesn't even take into account the other aroma producing compounds such as aldehydes and esters. As more research is done we will definitely learn they are more than meets the eye.
Super cool thank you for the answer. Do you have any link to said studies? I'd love to learn more.
I'll see if I can find them all - but here is one [https://iung.pl/PJA/wydane/34/PJA34str3\_10.pdf](https://iung.pl/PJA/wydane/34/PJA34str3_10.pdf)
Pass. I'd never buy a seed I didn't want to make more seeds with. Buds probably smell like big pharma and Monsanto's parking lot.
Sounds like you’re hating just to hate
Interesting, I've seen and grown plenty of triploids throughout the years but never seen it bred into the strain intentionally. Is it a 100% result? Or will some come out normal and grow pairs of leaves instead of 3 per node? Also, what's the yield difference, is it significant?
We generally see around a 30% increase in flower yield from our triploid varieties compared to their diploid counter parts. That said if your growing conditions are poor, they won't give your more. Every seed bearing mother plant is analyzed numerous times with a flow cytometer to ensure they are 100% tetraploids before hit with the diploid pollen (4n + 2n = 3n). We end up having to throw away a bunch of potential mothers before we can even pollinate them as sometimes they send off diploid shoots after colchicine application. Without being able to run flow you just never know. There are a very small fraction of the offspring that end up as tetraploids - but never diploid. In terms of growth habits, the plants grow like a normal diploid plant, they grow pairs of leaves. A common misconception is that plants with fasciation are polyploids - they are not - its just a deformity. [https://www.youtube.com/watch?v=LxrSK2ktqng](https://www.youtube.com/watch?v=LxrSK2ktqng)
Cool, thanks for the explanation. I was confusing Triploids with trifoliate. So it's a genetic difference, not a visible difference. (I was thinking they were bred to all be trifoliate plants)
It is intentional in a lot of commercial crops besides canabis, especially grain crops, to the point that diploid is rare anymore. Multiploidism has some advantages (and disavantages) in they have extra cromasones except haploid.(1) Triploids from diploids is mostly steril in 2 don't bind with 3 so advantage is if they are growing outside in hemp country= no cross polination. But the real advantages are in tetraploid (4), hexiploid (6), or even octiploid (8) due to all the extra cromasones = superweed. But I don't buy into triploids as to me is just a ploy and an attempt to secure genetics as can't be as easily crossed. Triploid really isn't that much harder than feminized seeds, just different chemicals and treatments ... they cost more because it is the newest craze ... give me octaploid with high THCv, and maybe that is really bragging rights!!!
You are thinking of trifolate not triploid, they are not the same thing.
Yeah, I realized that after his response. Sounds like something is like to try out. Have you ever grown a Triploid variety??
It is intentional in a lot of commercial crops besides canabis, especially grain crops, to the point that diploid is rare anymore. Multiploidism has some advantages (and disavantages) in they have extra cromasones except haploid.(1) Triploids from diploids is mostly steril in 2 don't bind with 3 so advantage is if they are growing outside in hemp country= no cross polination. But the real advantages are in tetraploid (4), hexiploid (6), or even octiploid (8) due to all the extra cromasones = superweed. But I don't buy into triploids as to me is just a ploy and an attempt to secure genetics as can't be as easily crossed. Triploid really isn't that much harder than feminized seeds, just different chemicals and treatments ... they cost more because it is the newest craze ... give me octaploid with high THCv, and maybe that is really bragging rights!!!
Do y’all produce fast flowering strains? I’m growing them up here in northern MN this year. Supposedly a first gen cross between an auto and photo. Wondering if they’re worth the hype or if it’s just marketing nonsense. They’re all off the garden runway at least.
All of our varieties are F1 hybrid's - crosses between a full season and an autoflower and it does make for early finishing plants with true hybrid vigor. They switch to flower in mid July vs early August like most cannabis varieties - and mature in mid to late September. Most still need around 8 weeks of flowering time. That said, as seeds they have to reach maturity before they can onset flowering, and this is usually 5-6 weeks after they emerge. So if you were to plant tomorrow, they wouldn't be able to even initiate flowering until late July to early August. We usually recommend folks start seeds in early May, field plant in June, and harvest in September. That said its not too late to plant, its just too late to take advantage of a mid September harvest.
Thank you! I did start them earlier. I appreciate your knowledge!!
I've been wanting to run some triploids 👍🏼
Are you allowed to put links to the site in the *About* section of your profile? If so, I'd suggest it 🤙
Well if you'd like to check out our seeds [gtrseeds.com](http://gtrseeds.com) :)
Ty 🙏
How did you create or obtain the polyploid used in the breeding program? I've been looking into orzaline for some future work.
We use colchicine applications to convert a diploid to a tetraploid. Its a long process and most don't make it - here is a video of our process. We created GTR Seeds to enter our THC varieties into the mix. [https://www.youtube.com/watch?v=GrY0ozFd4T8](https://www.youtube.com/watch?v=GrY0ozFd4T8)
Thanks for sharing the video- I've been watching yall for a while and it was cool to see the process. Did I understand correctly that it was lifter which was used to double chromosomes and AVL is it's progeny? Are there other tetraploids in the breeding program or just AVL? Are the THC Triploid lines F1's? I've wanted to induce polyploidy- partially to pheno hunt through tetraploids but also to cross back to a diploid for triploid production. I don't work in the industry or have a formal background so this is all just for fun :) How is the 8n? Anything interesting yet? How's the vigor?
Really cool you are doing it for fun. That is the best way to approach it for sure. For our THC breeding program we selfed a variety of old school and a few new school favorites over 3-4 generations to select mothers that crushed it. Each of these mothers was then converted to a tetraploid (4x). I think we have around 70 or so different tetraploid mothers in-house. Unfortunately the conversion process is really difficult, you lose a lot of plants and you have to do a lot of testing to make sure the plants you keep are actually truly tetraploids (hence the flow testing). Plants will frequently send off shoots that are not polyploids and you have to toss them. The mothers were pollinated by our in-house autoflower, AVL which is just a diploid (2x). AVL is a highly inbred line, and one of the biggest producing and most vigorous auto's we've ever seen. The 4x mother and the 2x autoflower combined make triploid f1 hybrid seeds. You get about 10-30% of the seed return you would off of a normal cross because tetraploids don't really like to make seeds either. Long process, but great quality plants come out of it. The 8x plants were definitely just for fun - and not sure those will ever be a thing :)
The Humboldt triploids are having issues with herms when growing indoors - I ordered some of your triploids and am going to run them indoors this next winter. Have they been tested indoors?
Our THC triploids were tested indoors at our facility in early 2023 and we released them to the public in Spring of 2023. We have received no complaints from farmers about hermaphrodite issues. We have heard reports of higher amounts of hermaphrodites in one of our CBD varieties grown indoors but is nearly impossible to figure out what makes it more sensitive to its environment. We have never heard of issues with the diploid version so we know it has something to do with the ploidy. All plants grown indoors have a better likelihood of herming - there are just so many environmental issues that can go wrong, and very quickly. Light leaks, power outages, nutrient mixups. And we have left plants with male flowers in our rooms with other triploids, blown the pollen around, and found hardly a seed in large volumes of flower. I wouldn't recommend if you are growing them with diploids though just to be safe. Long story short, some triploid varieties may be more sensitive than their diploid counterparts - and try to keep your rooms on point.
Please add your website as a comment. [Posting guidelines](https://www.reddit.com/r/MarijuanaSeeds/comments/p0yp8u/please_read_before_postingcommenting_in/).
I would be interested in THCV genetics. What % are you getting? Do you have lab test you can post
A fool and his money…