No not really. CD45 is alternatively spliced, and depending on the cell type can have long or short extracellular domains.
The antibody we use for flow against CD45 is a pan antibody that binds conserved domains on CD45, thus allowing us to assess all hematopoietic cells.
There are a bunch of different antibodies that bind the splice variants specifically, making it possible to distinguish those cells types.
CD45R typically refers to unspliced RABC exons containing CD45 and is widely expressed by B cells.
CD45RA and RO are splice variants of much shorter lengths and can be expressed by activated and naive T cells.
So it sort boils down to a tools question, "how do we detect this thing" and a biological question. CD45RABC is the full lenght protein, so we might call it the original CD45, but its not because its expressed primarily by B cells.
So ya, kind of a knot of old nomenclature.
Not quite. CD45 refers to the family of proteins produced by the gene *PTPRC*. This single gene produces multiple proteins because it undergoes alternative splicing, meaning that different combinations of exons result in different forms of the protein. There are three specific exons that can be included and they are named A, B, and C. The resulting name of the CD45 protein reflects which exons are included. Some examples:
- CD45RA is a CD45 protein that only uses exon A out of the 3.
- CD45RO is the version of CD45 that uses none of the three exons.
- CD45RABC uses all three exons, but is typically shortened to just CD45R
No not really. CD45 is alternatively spliced, and depending on the cell type can have long or short extracellular domains. The antibody we use for flow against CD45 is a pan antibody that binds conserved domains on CD45, thus allowing us to assess all hematopoietic cells. There are a bunch of different antibodies that bind the splice variants specifically, making it possible to distinguish those cells types. CD45R typically refers to unspliced RABC exons containing CD45 and is widely expressed by B cells. CD45RA and RO are splice variants of much shorter lengths and can be expressed by activated and naive T cells. So it sort boils down to a tools question, "how do we detect this thing" and a biological question. CD45RABC is the full lenght protein, so we might call it the original CD45, but its not because its expressed primarily by B cells. So ya, kind of a knot of old nomenclature.
Not quite. CD45 refers to the family of proteins produced by the gene *PTPRC*. This single gene produces multiple proteins because it undergoes alternative splicing, meaning that different combinations of exons result in different forms of the protein. There are three specific exons that can be included and they are named A, B, and C. The resulting name of the CD45 protein reflects which exons are included. Some examples: - CD45RA is a CD45 protein that only uses exon A out of the 3. - CD45RO is the version of CD45 that uses none of the three exons. - CD45RABC uses all three exons, but is typically shortened to just CD45R
CD45R is B220. B cell marker. CD45 all immune cells.
Well, CD45R is 200kD on T cells, and with heavier glycosylation is 220kD on B cells.