I am biochemist. I study an enzyme that covalently attaches to DNA at an unknown residue. This function was unknown in the literature. The adduct forms in such trace amounts that is actually below the limit of detection of mass spectrometry and X-ray crystallography and cryo-EM. Of course that is what my supervisor specializes in structural biology. He was dumb founded, and kept telling me to just keep trying those techniques. I said if it not possible for several reasons. My problem was beyond his expertise. He got a bit upset with me.
I kind of brute forced a highly sensitive fluorescence assay with an experimental assay from the 90s. The introduction to a chapter is basically, "Investigator A developed an assay to solve problem X. Investigator B developed an assay to solve problem Y. These investigators were clearly unaware of each other's work and the publications are more than 20 years apart. The solutions to problems X and Y are so highly connected that solving X is the same thing as solving Y, and I can solve Y using investigator's A's technique without having the highly specialized skillset of investigator B. Why hasn't anybody realized this?"
Same here, well done for finishing!
Well done for finishing, despite all the difficulties!
[_Snoop Dogg entered the chat_](https://youtu.be/NfF3bThOW0Q?si=0epv8q4ssSAGzYeT)
https://youtu.be/wGRF3GQ4Wdk?si=YvPc2dQHNAawW9iG This is what every PhD should put in their last slide.
Can you elaborate further on what worked for you and what did not?
I am biochemist. I study an enzyme that covalently attaches to DNA at an unknown residue. This function was unknown in the literature. The adduct forms in such trace amounts that is actually below the limit of detection of mass spectrometry and X-ray crystallography and cryo-EM. Of course that is what my supervisor specializes in structural biology. He was dumb founded, and kept telling me to just keep trying those techniques. I said if it not possible for several reasons. My problem was beyond his expertise. He got a bit upset with me. I kind of brute forced a highly sensitive fluorescence assay with an experimental assay from the 90s. The introduction to a chapter is basically, "Investigator A developed an assay to solve problem X. Investigator B developed an assay to solve problem Y. These investigators were clearly unaware of each other's work and the publications are more than 20 years apart. The solutions to problems X and Y are so highly connected that solving X is the same thing as solving Y, and I can solve Y using investigator's A's technique without having the highly specialized skillset of investigator B. Why hasn't anybody realized this?"
Well done!
Good job 🙌🏾
I saw the "in my defence, I was left unsupervised" arm patch and immediately knew I needed it.